I590:  Introduction to genomics for non-biologists (3CR)

Spring Semester 2005: Tuesday/Thursday, 9:30am-10:45pm, Eigenmann 921

Instructor: Haixu Tang

Description: We aim to introduce the broad frontiers of contemporary biology to the students who intend to work on biology-related problems but so far have no biology background.  This class will cover important themes in molecular genetics and cell biology, including


- biochemistry of proteins and nucleic acids;

- metabolic pathways;

- 3D structure of biological molecules;

- genetic mechanisms;
- internal organization of the cell: membrane, intracellular compartments and traffic, and the cell cycle;
- cell communiation;
- immune system;
- development of multicellular organisms;

- diseases;

- biotechnologies in genomics and proteomics.


This course is designed for non-biology-major graduate students working in the biology-related areas, particularly bioinformatics and computational biology. Therefore, we assume the students are mature students who want to learn biology contents as quickly as possible so they can return to their biology-related projects and collabrate with biologists smoothly. We expect after taking this class, the students will be able to (1) understand the main idea in biological literatures; (2) communicate with biologists with no difficulty; (3) write the summary of the biological essence in their biology-related projects without much help from biology collabrators.

 

Textbook: Alberts, Johnson, Lewis, Raff, Roberts, Walter: Molecular biology of the cell, 4th edition. This is an expensive book and we mainly use it as a reference. However, if you intend to continue working on biology-related problems, I recommend you buy this excellent textbook. Certainly you can also refer this book from its online version at NCBI website with a text searching fashion: Some of the topics from the course can not be found in this book. We will distribute complementary lecture notes and reading materials along the course for these topics. We also recommend the students to read the book Campbell, Reece and Simon, Essencial Biology, for a general idea of biology.

Assignments: We will have 4 take-home assignments.

Grading: One mid-term exam (30%), Combined assignments (30%), Final exam (40%).

Office hour: Tuesday and Thursday (11:00am-noon), or upon appointment.

Prerequisites:  No particular knowledge required, except high school level chemistry/biology and most important common sense.

Preliminary syllabus [This may change!]:


 

Week
Date
Contents
Lecture notes
1
1/11 Tue.
Introduction to the class and to molecular biology
MBC chap 1, slides

1/13 Thr.
Genetic variations
note, slides
2
1/18 Tue.
Biochemistry of biomolecules: proteins, nucleic acids, glycans, lipids, etc. MBC chap 2, slides

1/20 Thr.
Catalysis and the Use of Energy by Cells I
MBC chap 2, slides
3
1/25 Tue.
Catalysis and the Use of Energy by Cells II
(Homework 1)
MBC chap 2, slides

1/27 Thr.
Mendelian genetics I.
Notes: 1, slides
4
2/1 Tue.
Mendelian genetics I.
slides

2/3 Thr.
Mendelian genetics II.
Notes: 2. slides
5
2/8 Tue.
The Shape and structure of proteins
MBC chap 3, slides

2/10 Thr.
DNA, chromosomes and genomes
(Homework 1 due)
MBC chap 4, slides
6
2/15 Tue.
DNA replication, repair and recombination
(Homework 2)
MBC chap 5, slides

2/17 Thr.
DNA replication, repair and recombination
MBC chap 5, slides
7
2/22 Tue.
Central dogma: from genome to proteins (Prokaryotes)
MBC chap 6, slides

2/24 Thr.
Central dogma: from genome to proteins (Prokaryotes)
MBC chap 6, slides
8
3/1 Tue.
Central dogma: from genome to proteins (Eukaryotes)
(Homework 2 due)
MBC chap 6, slides

3/3 Thr.
Regulation of Gene expression: transcriptional regulation
MBC chap 7, slides
9
3/8 Tue.
Regulation of Gene expression: transcriptional regulation
MBC chap 7, slides

3/10 Thr.
Mid-term exam
Spring access (3/12-3/20)
11
3/22 Tue
Regulation of Gene expression: post-transcriptional regulation
MBC chap 7, slides

3/24 Thr.
Topics in biotechnology: 1. Genome sequencing and annotation
Genomes, chap 6, slides
12
3/29 Tue.
Topics in biotechnology: 2. Manipulating proteins, DNA and RNA
(Homework 3)
MCB chap 8, slides

3/31 Thr.
Topics in biotechnology: 2. Manipulating proteins, DNA and RNA
MCB chap 8, slides
13
4/5 Tue.
Topics in biotechnology: 3. Gene expression analysis note, slides

4/7 Thr.
Topics in biotechnology: 4. Proteomics: Mass spectrumetry and protein-protein interaction
(Homework 3 due)
note 1, 2, slides
14
4/12 Tue.
Cell membrane
(Homework 4)
MBC chap 10,11 slides

4/14 Thr.
Intracellular compartments and protein localization MBC chap 12, slides
15
4/19 Tue.
The cell cycle and the programmed cell death
MBC chap 17, slides

4/21 Thr.
Immunity
(Homework 4 due)
MBC chap 24, slides
16
4/26 Tue.
Development of multicellular organisms
MBC chap 21, slides

4/28 Thr.
Cancer and infectious disease
MBC chap 23,35, slides
17
5/2 Tue.
Final report due